Goat anti-Rabbit IgG (Heavy & Light Chain) Antibody (Agarose Beads)

Catalog No. ABIN233744

Supplier Product No.: 211-1150

Quick Overview for Goat anti-Rabbit IgG (Heavy & Light Chain) Antibody (Agarose Beads) (ABIN233744)

Target: IgG

Clonality: Polyclonal

Conjugate: Agarose Beads

Application: Immunoprecipitation (IP)

Isotype: IgG

Product Details

Binding Specificity: Heavy & Light Chain

Reactivity: Rabbit

Host: Goat

Supplier Product No.: 211-1150

Supplier: Rockland

Purpose: Rabbit IgG Secondary Antibody Agarose

Cross-Reactivity (Details): Sufficient antibody capacity is provided to bind a minimum of 5 mg of pure Rabbit IgG.

Characteristics: Anti-Rabbit IgM antibody generated in goat specifically detects rabbit IgM mu heavy chain.

Purification: This product is an IgG fraction antibody coupled to activated agarose.

Application Details

Application Notes: Application Note: Anti Rabbit IgG Secondary Antibody Agarose can be used for immunoaffinity chromatography of polyclonal or monoclonal rabbit IgG antibodies. Once bound, non-specific antibodies are removed by copious washing followed by elution of specific antibody at low pH . Immunoprecipitation Dilution: User Optimized

Restrictions: For Research Use only

Handling

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

, Preservative:0.1 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store vial at 4° C prior to opening. DO NOT FREEZE.

Expiry Date: 12 months

Target Details

Target: IgG

Target Type: Antibody

Background: It is a protein complex composed of four peptide chains - two identical heavy chains and two identical light chains arranged in a Y-shape typical of antibody monomers. Each IgG has two antigen binding sites. Representing approximately 75 % of serum immunoglobulins in humans, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.